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Fluorescent Probes（熒光探針）—cayman品牌推薦

更新時(shí)間：2022-05-10   點(diǎn)擊次數(shù)：742次

Cayman擁有500多種熒光探針，幫助您提升研究中所需的多功能性、靈敏度和定量能力。這一系列探針能夠檢測(cè)細(xì)胞內(nèi)活動(dòng)、蛋白質(zhì)相互作用、一系列酶活性和許多其他重要目標(biāo)蛋白。為實(shí)驗(yàn)選擇最合適的熒光染料請(qǐng)參考以下cayman熒光探針表格。


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檢測(cè)細(xì)胞活力和細(xì)胞增殖的探針

Item No.Product NameDetectsCell Permeable?

Excitation 

Maximum (nm)

Emission

Maximum (nm)

113977-Aminoactinomycin DDNA; non-viable cells×488,546,578650
14948Calcein AM*cell viability√494517
20637Calcein Blue AM*cell viability√360445
20632Calcein Red™ AM*cell viability√560574
20639Calcein UltraBlue™ AM*cell viability (higher photostability and stronger fluorescence intensity atphysiological pH than Calcein Blue)√360445
14456CFDA-SEcell proliferation√491518
20698CytoTrace™ Red CMTPXcell division; cell tracking√577602
35701Fluorescein diacetatecell viability√490520
19583Green CMFDAcell division; cell tracking√492517
15003JC-1mitochondrial integrity; apoptosis√485(monomer)
520-570(aggregate)		535(monomer)
570-610(aggregate)
14289Propidium IodideDNA; RNA; non-viable cells×488-535617
14322Resazurin (sodium salt)enzyme activity; cell viability√570580
21426Tetramethylrhodamine ethyl ester (perchlorate)mitochondrial function; cell viability√550575

*Also available: membrane-impermeable non-AM forms

View a complete list of cell viability fluorescent probes at 


檢測(cè)核苷酸的探針

Item No.Product NameDetectsCell Permeable?

Excitation

Maximum (nm)

Emission

Maximum (nm)

14338Acridine OrangeNucleic acids; pH√502(dsDNA)
460(ssDNA andRNA) 
475 (low pH)		525(dsDNA)
650(ssDNA and RNA) 
590(low pH)
224399-Amino-6-chloro-2- methoxyacridineDNA and pH gradients; poly(dA-dT) sequences of DNA√411475
11315Chromomycin A3DNA (two or more contiguous GC base pairs)×445575
14285DAPI (hydrochloride)DNA (AT-rich sequences)×358461
15547Hoechst 33342 (hydrochloride)DNA (AT-rich sequences); nuclear marker√350461
14488Pyronin YdsRNA√540-550560-580

View a complete list of DNA fluorescent probes at 


檢測(cè)氧化應(yīng)激和活性反應(yīng)組分的探針

Item No.Product NameDetects

Excitation

Maximum (nm)

Emission 

Maximum (nm)

10157APFhydroxyl radical; hypochlorite ion; certain peroxidase intermediates490515
27086C11 BODIPY 581/591lipid oxidation581/500(oxidized)591/510(oxidized)
14051Coumarin Boronic Acidperoxynitrite; hypochlorous acid; hydrogen peroxide332470
19111Coumarin hydrazinereactive carbonyl groups365430/550
(upon reaction with carbonyls)
85165DAF-2 diacetatenitric oxide485538
18767DAF-FM diacetatenitric oxide495515
851552,7-Dichlorodihydrofluorescein diacetateperoxynitrite formation502523
12013Dihydroethidiumsuperoxide490590
85100Dihydrorhodamine 123peroxynitrite formation500536
62237DPPPhydroperoxides; low-density lipoprotein; cellular oxidation351380
345647-Fluoro-2,1,3-benzoxadiazole- 4-sulfonate (ammonium salt)thiols380515
14872Lucigeninsuperoxide; chloride455505
16803Luminolperoxidation; iron; gold; cyanide355411
17097Monobromobimanereactive sulfur; thiol groups398490
10005983Pentafluorobenzenesulfonyl fluoresceinhydrogen peroxide465-505505-555
16929WSP-5hydrogen sulfide502525

 View a complete list of fluorescent probes for oxidative stress and reactive species detection at 


檢測(cè)細(xì)胞骨架蛋白的探針

Item No.Product NameDetects

Excitation

Maximum (nm)

Emission 

Maximum (nm)

210169-(2,2-Dicyanovinyl)julolidinetubulin and other cytoskeletal proteins450480(low viscosity solvents) 
505(high viscosity solvents)
20546Phalloidin-California Red™ ConjugateF-actin583605
20478Phalloidin-Fluorescein ConjugateF-actin492518
20547Phalloidin-iFluor™ 350 ConjugateF-actin353442
20548Phalloidin-iFluor™ 405 ConjugateF-actin400421
20549Phalloidin-iFluor™ 488 ConjugateF-actin493517
20552Phalloidin-iFluor™ 555 ConjugateF-actin556574
20553Phalloidin-iFluor™ 594 ConjugateF-actin590618
20554Phalloidin-iFluor™ 633 ConjugateF-actin634649
20555Phalloidin-iFluor™ 647 ConjugateF-actin650665
20545Phalloidin-Tetramethylrhodamine ConjugateF-actin546575

View a complete list of cytoskeletal protein fluorescent probes 


Fluorescent Probes（熒光探針）—cayman品牌推薦Fluorescent Probes（熒光探針）—cayman品牌推薦Fluorescent Probes（熒光探針）—cayman品牌推薦
Actin filaments stained with
Phalloidin-iFluor™ 350 Conjugate		HeLa cells stained with
Phalloidin-iFluor™ 488 Conjugate		HeLa cells labeled with
Phalloidin-iFluor™ 594 Conjugate



蛋白酶底物探針

Item No.Product NameDetects

Excitation

Maximum (nm)

Emission 

Maximum (nm)

9002021Abz-Val-Ala-Asp-Nva-Arg-Asp- Arg-Gln-EDDnp (trifluoroacetate salt)human proteinase 3 activity320420
26640Ac-ANW-AMCβ5i/LMP7 subunit of the 20S immunoproteasome activity340-360440-460
14986Ac-DEVD-AMCcaspase-3 activity340-360440-460
17051Ac-LEHD-AFC (trifluoroacetate salt)caspase-4, -5, and -9 activity400505
14991Ac-LETD-AFCcaspase-8 activity400505
14988Ac-VDVAD-AFCcaspase-2 activity400505
14992Ac-WEHD-AFC (trifluoroacetate salt)caspase-1, -4, and -5 activity400505
17591Ac-YVAD-AFCcaspase-1 and -4 activity400505
27580Gly-Arg-AMC (hydrochloride)cathepsin C activity340-360440-460
14907MeOSuc-Ala-Ala-Pro-Val-AMChuman leukocyte and porcine pancreatic elastase activity355-380440-460
10008119Suc-Leu-Leu-Val-Tyr-AMC20S proteasome, chymotrypsin-like proteases, and calpain activity360460
11675(Z-Ala-Ala-Ala-Ala)2Rh110elastase activity485525
10008117Z-LLE-AMCcaspase-like activity of the 26S proteasome or 20S proteolytic core340-360440-460

 View a complete list of protease activity fluorescent probes at 


其他酶底物探針

Item No.Product NameDetects

Excitation

Maximum (nm)

Emission

Maximum (nm)

629107-hydroxycoumarinyl ArachidonatecPLA2 activity335450
25583C12FDGβ-galactosidase activity485530
35500DiFMUPphosphatase activity358455
165514-Methylumbelliferyl-α-D- Galactopyranosideα-galactosidase activity

330(pH 4.6);

370 (pH 7.4);

385(pH 10.4)		445-454
209484-Methylumbelliferyl-β-D- Glucopyranosideβ-glucosidase and β-glucocerebrosidase activity

330(pH 4.6);

370 (pH 7.4);

385(pH 10.4)		445-454
195434-Methylumbelliferyl-α-L- Iduronide (free acid)α-L-iduronidase activity320(pH 1.97-6.72);
360(pH 7.12-10.3)		445-455
(increases as pH decreases)
195244-Methylumbelliferyl 6-thio- Palmitate-β-D-Glucopyranosidepalmitoyl-protein thioesterase (PPT/CLN1) activity320(pH 1.97-6.72);
360(pH 7.12-10.3)		445-455
(increases as pH decreases)

 View a complete list of enzyme substrate fluorescent probes at 


檢測(cè)蛋白交互作用探針

Item No.Product NameDetects

Excitation

Maximum (nm)

Emission 

Maximum (nm)

206723-BODIPY-propanoylaminocaproic Acid N-hydroxysuccinimide esterproteins immobilized on PVDF membranes300-360,504511
20704FlAsH-EDT2proteins with a tetracysteine sequence508528
19767ReAsH-EDT2proteins with a tetracysteine sequence593608

View a complete list of probes to detect protein interactions at 


檢測(cè)鈣離子探針

Item No.Product NameDetects

Cell

 Permeable?

 Excitation

Maximum (nm)

Emission

Maximum (nm)

20424BTC AM*intracellular calcium (low affinity)√401(Ca2+-bound)/480529
35758Fluo-2 AM*intracellular calcium√490515
14960Fluo-3 AM*intracellular calcium√488526
31143Fluo-4 AM*intracellular calcium√490520
14591Fura-2 AM*intracellular calcium√380 (low [Ca2+])
340 (high [Ca2+])		510
34993Fura-2 Leakage Resistant AM*intracellular calcium√340 (Ca2+-bound)
380 (Ca2+-free)		505
20416Fura-FF AM*intracellular calcium (low affinity)√365 (no Ca2+)
339 (high [Ca2+])		514(no Ca2+)
507(high [Ca2+])
34985ICR-1 AM*intracellular calcium√580660
20417Indo-1 AM*intracellular calcium√349-364475-485(no Ca2+)
400-410(Ca2+-bound)
20442Rhod-5N AM*intracellular calcium (low affinity)√551576
20444Rhod-FF AM*intracellular calcium (low affinity)√552580

*Also available: membrane-impermeable non-AM forms

View a complete list of calcium fluorescent probes at 


檢測(cè)pH值探針

Item No.Product NameDetectsCell Permeable?

Excitation

Maximum (nm)

Emission

Maximum (nm)

17183BCECFpH× 440 / 490535
15922BCECF AMintracellular pH√ 440 / 490535


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