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更新時(shí)間:2022-07-12
FLuc mRNA表達(dá)一種熒光素酶蛋白,該蛋白最初是從螢火蟲(chóng)體內(nèi)分離出來(lái)的。熒光素酶通常用于動(dòng)物細(xì)胞培養(yǎng)中的基因表達(dá)和細(xì)胞活性檢測(cè)。在熒光素底物的存在下能夠發(fā)光。
CleanCap® FLuc mRNA (5moU) - (L-7202)
螢火蟲(chóng)熒光素酶mRNA (Trilink cat#L-7202)
產(chǎn)品描述
FLuc mRNA表達(dá)一種熒光素酶蛋白,該蛋白最初是從螢火蟲(chóng)體內(nèi)分離出來(lái)的。熒光素酶通常用于動(dòng)物細(xì)胞培養(yǎng)中的基因表達(dá)和細(xì)胞活性檢測(cè)。在熒光素底物的存在下能夠發(fā)光。
該mRNA使用Trilink公司專/利加帽法cleancap產(chǎn)生的天然的cap1帽結(jié)構(gòu),cleancap的加帽效率較高。該mRNA具有PolyA尾巴,且經(jīng)過(guò)5-甲氧基尿苷修飾,針對(duì)哺乳動(dòng)物系統(tǒng)進(jìn)行了優(yōu)化,和天然成熟的mRNA相似。
產(chǎn)品細(xì)節(jié)
產(chǎn)品貨號(hào) | L-7202 |
純化方式 | Passes Agarose Gel Mobility |
長(zhǎng)度 | 1929 nucleotides |
堿基組成 | Fully substituted with 5-Methoxy-U |
濃度 | 1.0 mg/mL |
溶液 | 1 mM Sodium Citrate pH 6.4 |
轉(zhuǎn)換因子 | 40 µg/OD260 |
推薦儲(chǔ)存條件 | At or below -40°C |
應(yīng)用 | Reporter Genes |
加帽結(jié)構(gòu) | AG Start, Cap 1, CleanCap |
其他名稱 | CleanCap®Firefly Luciferase mRNA (5-methoxyuridine) |
參考文獻(xiàn):
References
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Kim, J;Jozic, A;Sahay, G; . Naturally Derived Membrane Lipids Impact Nanoparticle-Based Messenger RNA Delivery
Gambari, R;Gasparello, J;Fabbri, E;Borgatti, M;Tamanini, A;Finotti, A; . Peptide Nucleic Acids for MicroRNA Targeting
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